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Detection by Rt-Pcr and Molecular Characterization of Tremovirus A Obtained from Clinical Cases of Avian Encephalomyelitis (AE) Outbreaks in Brazil Rev. Bras. Ciênc. Avic.
Torre,D De la; Nuñez,LF; Parra,SHS; Astolfi-Ferreira,CS; Ferreira,AJP.
ABSTRACT This study determined the presence of Tremovirus A as the possible agent related to Avian Encephalomyelitis in broiler chicks from the states of São Paulo (SP) Paraná (PR), Goiás (GO), Santa Catarina (SC) and Rio Grande do Sul (RS), between the years 2006 and 2015. Samples of the nervous, digestive, respiratory, immune, and renal systems, plus muscular organs from broiler chicks with neurological problems such as ataxia and muscle tremors, and four (4) commercial vaccines as positive control, were tested by reverse-transcriptase (RT-PCR) amplification and DNA sequencing. A highly conserved region (P1) of the viral genome, was used to amplify a segment which encodes a structural protein VP4. Out of 112 samples, 46 were positive (42%) for Tremovirus...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chicken; Tremovirus A; Avian Encephalomyelitis; Detection; Molecular characterization.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000300527
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Epidemiology of Avian Infectious Laryngotracheitis with Special Focus to South America: an update Rev. Bras. Ciênc. Avic.
Parra,SHS; Nuñez,LFN; Ferreira,AJP.
ABSTRACT Avian Infectious laryngotracheitis (AILT) is a respiratory tract disease of great importance because it causes significant economic losses in the poultry industry around the world. It is caused by a Gallid herpesvirus type 1, a member of the genus Iltovirus. The target system for Avian Infectious Laryngotracheitis virus (AILTV) infections is the respiratory system, and the main organ in which the virus remains latent is the trigeminal ganglia. However, the virus has demonstrated tropism for other organs besides the respiratory tract. The main transmission routes are ocular and respiratory. Infected birds with clinical symptoms are main sources of transmission, but birds with latent infections, litter, and contaminated fomites may also transmit the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Avian infectious laryngotracheitis; Biosecurity; Epidemiology; South America; Chicken.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000400551
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Molecular Diagnostic of Chicken Parvovirus (ChPV) Affecting Broiler Flocks in Ecuador Rev. Bras. Ciênc. Avic.
Torre,D De la; Nuñez,LFN; Puga,B; Parra,SHS; Astolfi-Ferreira,CS; Ferreira,AJP.
ABSTRACT Enteric diseases affect poultry and cause important economic losses in many countries worldwide. Avian parvovirus has been linked to enteric conditions, such as malabsorption and runting-stunting syndrome (RSS), characterized by diarrhoea, and reduced weight gain and growth retardation. In 2013 and 2016, 79 samples were collected from different organs of chickens in Ecuador that exhibited signs of diarrhea and stunting syndrome, and analysed for the presence of chicken parvovirus (ChPV). The detection method of ChPV applied was Polymerase Chain Reaction (PCR), using primers designed from the conserved region of the viral genome that encodes the non-structural protein NS1. Out of the 79 samples, 50.6% (40/79) were positive for ChPV, and their...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chicken parvovirus; Enteric diseases; Molecular diagnostic; PCR.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000400643
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Occurrence of infectious Laryngotracheitis Virus (ILTV) in 2009-2013 in the State of São Paulo - Brazil Rev. Bras. Ciênc. Avic.
Parra,SHS; Nuñez,LFN; Astolfi-Ferreira,CS; Ferreira,JP.
Infectious laryngotracheitis is a very important respiratory disease because it causes significant economic losses in the poultry industry. The target of ILTV infections is the respiratory system, and the main organ in which the virus remains latent is the trigeminal ganglia. However, the virus has demonstrated tropism for other organs as well. The present study was conducted to determine the presence of Infectious Laryngotracheitis Virus (ILTV) in the state of São Paulo. Samples submitted to LABOR- USP during the last four years (2009-2013) analyzed by a nested/PCR technique. Out of the 682 samples from layers tested for LTIV, 12.46 % were positive, and derived from in both traditional (trachea and trigeminal ganglion) and untraditional (cecal tonsils,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Infectious Laryngotracheitis; PCR; Detection; Trachea; Trigeminal.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2015000100117
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